در مورد بیماری ها و هورمونها فایل اکسل دارم که باید طبق فایلی که ارسال کردم انجام بشه و نتیجه و گراف بده بهم

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1-Data Preprocess
Imputation of missing values: Replace zeros with a small value
use which method to do this
could also do a compare results with different approaches
Log transformation: base e
scaling
normalizationing: Normalization z-values (Quantil)
Data Filtering: Remove noise and low-quality data points.
 
 
2- Statistical Analysis
Multivariate Analysis:
PCA (Principal Component Analysis): Reduce dimensionality and identify patterns.
PLS-DA (Partial Least Squares Discriminant Analysis): Classify samples and identify discriminant metabolites.
 
 
3- Univariate Analysis:
T-tests: Compare metabolite levels between two groups.
ANOVA: Compare metabolite levels among multiple groups.
Correlation Analysis: Assess relationships between metabolites.
 
• 4. Validation
o Biological Replicates: Use independent biological replicates to validate findings.
o Technical Replicates: Repeat measurements to ensure reproducibility.
o External Validation: Confirm results using different analytical methods or datasets.
• 5. Reporting and Visualization
o Visualization Tools: Use heatmaps, volcano plots, pathway maps, and box plots to visualize data.
o Statistical Reporting: Report statistical methods and significance levels clearly.
o Data Sharing: Share raw and processed data in repositories like MetaboLights or the Metabolomics Workbench.
• ? Cluster Profiles / Fold change

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کاربر 604825-233

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